LOCUS       sequence-53955-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1847_2350
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccagg
      121 ctgaccatcc ccatctccca gattgcaccc gaccttatcc tcgctgacct gcctcgcaac
      181 atcatgctga acaatgacga gctggagttt gaacaagcac ccgagttcct gctaggcgac
      241 ggctctttcg gttcagttta ccgtgctgct tacgagggtg aagaggttgc cgtgaagatt
      301 tttaacaagc acacttcgct ccgtctgctg cgtcaggagc ttgtggttct ctgccacctg
      361 caccaccctt ccctgatctc cctgctggcc gccggcatta ggcctcgcat gctagtgatg
      421 gaactggcca gtaagggttc attggaccgc ctgctgcagc aggacaaggc tagcctgacc
      481 cgtacgctcc agcaccgtat cgccctgcat gtggccgacg gactgcgcta cttgcacagt
      541 gccatgatca tttatcgcga tctgaagcct cataatgttc ttctgttcac gctctaccca
      601 aacgcagcta tcattgccaa aattgccgat tacggcatag cccagtactg ctgtcgtatg
      661 ggtatcaaga ctagcgaggg tacacctggg ttccgcgctc ccgaagtcgc acgcgggaac
      721 gtgatctaca accagcaggc cgacgtgtac tcgtttggcc tgctcctgta tgacattctc
      781 actaccggag gccggattgt ggagggcctg aaatttccca acgagttcga cgagctggag
      841 atccagggca agctgcctga ccccg
//
