LOCUS       sequence-53957-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1847_2232
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..92
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             93..110
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatatag
       61 gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc aggctgacca
      121 tccccatctc ccagattgca cccgacctta tcctcgctga cctgcctcgc aacatcatgc
      181 tgaacaatga cgagctggag tttgaacaag cacccgagtt cctgctaggc gacggctctt
      241 tcggttcagt ttaccgtgct gcttacgagg gtgaagaggt tgccgtgaag atttttaaca
      301 agcacacttc gctccgtctg ctgcgtcagg agcttgtggt tctctgccac ctgcaccacc
      361 cttccctgat ctccctgctg gccgccggca ttaggcctcg catgctagtg atggaactgg
      421 ccagtaaggg ttcattggac cgcctgctgc agcaggacaa ggctagcctg acccgtacgc
      481 tccagcaccg tatcgccctg catgtggccg acggactgcg ctacttgcac agtgccatga
      541 tcatttatcg cgatctgaag cctcataatg ttcttctgtt cacgctctac ccaaacgcag
      601 ctatcattgc caaaattgcc gattacggca tagcccagta ctgctgtcgt atgggtatca
      661 agactagcga gggtacacct gggttccgcg ctcccgaagt cgcacgcggg aacgtgatct
      721 acaaccagca ggccgacgtg tactcgtttg gcctgctcct gtatgacatt ctcactaccg
      781 gaggccggat tgtggagggc ctgaaatttc ccaacgagtt cgacgagctg gagatccagg
//
