LOCUS       sequence-53959-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1500_2232
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..101
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             102..119
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catctcgcaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
       61 taaatatagg atctatgcac caccatcacc accatcacca cttggtgccc cgcggatcct
      121 tacgtaagac cattatcaac gaaagcctga acttcaaaat tcgcgaccag ctggtggtcg
      181 gacagctgat ccccgactgc tacgtggaac ttgagaagat catcctgagc gagcgcaaga
      241 atgtgcctat cgagtttcca gtgatcgatc gcaaacgtct cctccagctc gtgcgtgaga
      301 atcagctgca gctcgacgag aacgagctgc ctcatgctgt gcacttcctt aacgagtcag
      361 gtgtcctcct gcacttccag gacccggctc tccagctgag tgacctctat ttcgtggagc
      421 caaagtggct ctgtaaaatt atggctcaaa tcctcactgt caaggtagag ggctgtccaa
      481 agcacccaaa gggcatcatc tcgcgtcgcg acgtggaaaa gtttctctct aagaagcgca
      541 agtttcccaa gaactacatg agccagtact ttaaactgtt ggagaagttc cagatcgctc
      601 tgcctatcgg cgaagagtac ctgttggttc cttcctccct gtccgatcac cgccccgtga
      661 ttgagctgcc tcactgcgag aacagcgaga ttatcattcg cctatacgag atgccgtact
      721 ttccgatggg cttctggtca aggctcatca atcgtctgct tgaaatctcc ccatatatgc
      781 tgagcggtcg tgaacgcgct ctgaggccaa accggatgta ctggcgccag gggatttact
      841 tgaactggtc ccctgaggct tattgtctgg tgggctcgga agtcctggat aaccaccccg
      901 aatcattcc
//
