LOCUS       sequence-53960-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DNPEP
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tcttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgctagagaa gggaacagct
      181 caaagaagcc cttgaagagg gtgagggtct ggaggactcc tgtggtgcag gccatctccc
      241 ggatagagtg catggccagt tgggggctgc ctaaatccag cacccgcagc cccagccgag
      301 aagccaagat aggtccaatg gtggttccac agggggtgtc attccggacc atgagatcct
      361 gcagggggac cttgactttg ttggccacct ctcggatcag ggcctctgac accgcgtttg
      421 aagcatagcg ttgcttgctg ttcaccttga tcacggggcc cttgtggaat aaaggccggt
      481 ggttctcctc atgcttgtcc aggtagttgg gatgcacagc atgggccatg tctgcgctga
      541 tcatgaagga cttgggtatg gcttcctcga aggctgtcgg gtgctggcac gaggctgaga
      601 tccgccgcag caccagctct gtcagcagtg actgtgctcc ctgtgcactc tcagacccca
      661 cctcttcgtt gtcatagagt gtgaccatgc gcacgtgagg ctctgtggcc agggagccag
      721 ggcctgcaca ggaatctatc aaggcctgca gggcacagaa gcagctgtgc agattgtcca
      781 gccgaggagc aaagatgaac tcatcatagg caccacccaa gaccgcaggc tgggtgtctg
      841 caaggcagag ctccatctcc actatgtcct tggggctcag ccccagatgg gcacagagca
      901 gggacatgag gaccgaatgg tgccgctcat ccacagcatt gagaggccct ggctcaggag
      961 tccccttctc cagctcctcc tggatggctg tggcaagaat ggggactaga tgcatctctg
     1021 tgt
//
