LOCUS       sequence-53964-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1B3
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             784..804
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             805..822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             823..846
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             896..913
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 accttaagaa ggagatatac tatgcacaag cgtccggttc cggccaaacg ctatgacaac
      181 gtgactattc tgttctccgg tatcgtaggg tttaatgcat tttgtagcaa acatgcgtcg
      241 ggcgagggtg ctatgaagat tgttaacctg ctgaatgatc tgtacacacg tttcgatacg
      301 ctgaccgact ctcgcaaaaa cccgtttgtg tataaagtcg aaactgttgg cgataagtat
      361 atgacagtga gtggtctgcc ggagccgtgc atccatcacg cccgttcaat ttgtcatctg
      421 gcactggaca tgatggaaat tgcggggcag gtacaggtcg atggcgaaag cgttcaaatc
      481 acgattggta tccacaccgg ggaggtggta actggcgtca ttggtcagcg catgccgcgt
      541 tactgcctgt tcggcaatac ggttaacctg acctcccgca cagaaaccac tggtgaaaaa
      601 gggaaaatca atgtgtcgga gtatacatac cgttgtctga tgtctccgga aaacagtgac
      661 ccgcaatttc atctggagca ccgcggcccg gtaagcatga agggtaaaaa agaaccgatg
      721 caggtctggt tcctgtcacg taagaatacg ggcaccgaag agactaaaca ggatgatgac
      781 gcagagaacc tctacttcca atcgcaccat catcaccacc atgattacaa ggatgacgac
      841 gataagtgag gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca
      901 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      961 tgccacc
//
