LOCUS       sequence-53965-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1B3
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(91..114)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(115..132)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(133..153)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    839..863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(863..882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(864..882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtcgacgg agctcgaatt cggatcctca cttatcgtcg tcatccttgt aatcatggtg
      121 gtgatgatgg tgcgattgga agtagaggtt ctctgcgtca tcatcctgtt tagtctcttc
      181 ggtgcccgta ttcttacgtg acaggaacca gacctgcatc ggttcttttt tacccttcat
      241 gcttaccggg ccgcggtgct ccagatgaaa ttgcgggtca ctgttttccg gagacatcag
      301 acaacggtat gtatactccg acacattgat tttccctttt tcaccagtgg tttctgtgcg
      361 ggaggtcagg ttaaccgtat tgccgaacag gcagtaacgc ggcatgcgct gaccaatgac
      421 gccagttacc acctccccgg tgtggatacc aatcgtgatt tgaacgcttt cgccatcgac
      481 ctgtacctgc cccgcaattt ccatcatgtc cagtgccaga tgacaaattg aacgggcgtg
      541 atggatgcac ggctccggca gaccactcac tgtcatatac ttatcgccaa cagtttcgac
      601 tttatacaca aacgggtttt tgcgagagtc ggtcagcgta tcgaaacgtg tgtacagatc
      661 attcagcagg ttaacaatct tcatagcacc ctcgcccgac gcatgtttgc tacaaaatgc
      721 attaaaccct acgataccgg agaacagaat agtcacgttg tcatagcgtt tggccggaac
      781 cggacgcttg tgcatagtat atctccttct taaggttaaa caaaattatt tctagagggg
      841 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      901 ctcgatcctc tacacccgga
//
