LOCUS       sequence-53969-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MMAA
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
      181 gggtaccgag aacctgtact tccaatccat gaaggaccac acagaaggac tttctgataa
      241 agagcaaaga tttgtggata aactttatac tggtttaatc caagggcaaa gggcctgttt
      301 agcagaggcc ataactcttg tagaatcaac tcacagcagg aaaaaggagt tagcccaggt
      361 gcttcttcag aaagtattac tttaccacag agaacaagaa caatcaaata aaggaaaacc
      421 actagcattt cgagtaggat tgtctgggcc ccctggtgct ggaaaatcaa catttataga
      481 atattttgga aaaatgctta ctgagagagg gcacaaatta tctgtgctag ctgtggaccc
      541 ttcttcttgt actagtggtg gatcactctt aggtgataaa acccgaatga ctgagttatc
      601 aagagatatg aatgcataca tcaggccatc tcctactaga ggaactttag gaggcgtgac
      661 aaggaccaca aatgaagcta ttctgttgtg tgaaggagcg ggatatgaca taattcttat
      721 tgaaaccgtt ggtgtgggtc agtcggagtt tgctgttgct gacatggttg acatgtttgt
      781 tttactactg ccaccagcag gaggagatga gctgcagggt atcaaaaggg gtataatcga
      841 gatggcagat ctggtagctg taactaaatc tgatggagac ttgattgtgc cagctcgaag
      901 gatacaagcg gaatatgtga gtgcactgaa attactccgc aaacgttcac aagtctggaa
      961 accaaaggta attcg
//
