LOCUS       sequence-53970-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHF8
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             173..499
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             506..526
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtatgagcga
      181 taaaattatt cacctgactg acgacagttt tgacacggat gtactcaaag cggacggggc
      241 gatcctcgtc gatttctggg cagagtggtg cggtccgtgc aaaatgatcg ccccgattct
      301 ggatgaaatc gctgacgaat atcagggcaa actgaccgtt gcaaaactga acatcgatca
      361 aaaccctggc actgcgccga aatatggcat ccgtggtatc ccgactctgc tgctgttcaa
      421 aaacggtgaa gtggcggcaa ccaaagtggg cgcactgtct aaaggtcagt tgaaagagtt
      481 cctcgacgct aacctggccg gtaccgagaa cttgtacttc caatccatgc cagtgaagac
      541 cgggagccct acgttcgtca gagagctccg gagtaggact tttgacagct cagatgaagt
      601 gattctgaag cccactggaa atcaactgac cgtggaattc ctggaagaaa atagcttcag
      661 tgtgcccatc ctggtcctga agaaggatgg gttgggcatg acgctgccct cgccatcatt
      721 cactgtgagg gatgttgaac actatgttgg ttctgacaaa gagattgatg tgattgatgt
      781 gacccgccag gctgactgca agatgaagct tggtgatttt gtgaaatact attacagcgg
      841 gaagagggag aaagtcctca atgtcattag tttggaattc tctgatacca gactttctaa
      901 ccttgtggag acac
//
