LOCUS       sequence-53972-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2350
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..90
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             91..108
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatatagga
       61 tctatgcacc accatcacca ccatcaccac ttggtgcccc gcggatccaa gcacattggc
      121 tgtaaagcaa aggatattat tcgtttcctg cagcagcgtc ttaagaaggc cgtgccctac
      181 aatcgcatga agctgatgat cgtcgggaac accggttctg gaaagacaac cctcttgcag
      241 cagcttatga agactaagaa gtccgacttg gggatgcaga gcgccactgt ggggatcgac
      301 gtgaaagact ggccaatcca aatccgtgac aagcgcaagc gtgacttagt actgaacgtg
      361 tgggactttg ccggcaggga agagttctac tctacgcatc ctcacttcat gactcagcgt
      421 gccctctatc tggcagttta cgacctctca aagggccagg ctgaggtgga cgccatgaaa
      481 ccctggctgt ttaacatcaa ggctcgtgct tccagctccc ctgtgatctt agtcgggaca
      541 cacttggatg tttctgatga gaagcagcgt aaggcatgta tgagtaagat caccaaagag
      601 ctgttgaaca aacgcggctt tcccgccatc cgtgattacc acttcgtcaa cgctaccgag
      661 gaaagcgacg cactggccaa gttacgtaag accattatca acgaaagcct gaacttcaaa
      721 attcgcgacc agctggtggt cggacagctg atccccgact gctacgtgga acttgagaag
      781 atcatcctga gcgagcgcaa gaatgtgcct atcgagtttc cagtgatcga tcgcaaacgt
      841 ctcctccagc tcgtgcgtga gaatcagctg cagctcgacg agaacgagct gcctcatgct
      901 gtgcacttcc ttaacgag
//
