LOCUS       sequence-53974-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2232
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..100
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             101..118
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
       61 aaatatagga tctatgcacc accatcacca ccatcaccac ttggtgcccc gcggatccaa
      121 gcacattggc tgtaaagcaa aggatattat tcgtttcctg cagcagcgtc ttaagaaggc
      181 cgtgccctac aatcgcatga agctgatgat cgtcgggaac accggttctg gaaagacaac
      241 cctcttgcag cagcttatga agactaagaa gtccgacttg gggatgcaga gcgccactgt
      301 ggggatcgac gtgaaagact ggccaatcca aatccgtgac aagcgcaagc gtgacttagt
      361 actgaacgtg tgggactttg ccggcaggga agagttctac tctacgcatc ctcacttcat
      421 gactcagcgt gccctctatc tggcagttta cgacctctca aagggccagg ctgaggtgga
      481 cgccatgaaa ccctggctgt ttaacatcaa ggctcgtgct tccagctccc ctgtgatctt
      541 agtcgggaca cacttggatg tttctgatga gaagcagcgt aaggcatgta tgagtaagat
      601 caccaaagag ctgttgaaca aacgcggctt tcccgccatc cgtgattacc acttcgtcaa
      661 cgctaccgag gaaagcgacg cactggccaa gttacgtaag accattatca acgaaagcct
      721 gaacttcaaa attcgcgacc agctggtggt cggacagctg atccccgact gctacgtgga
      781 acttgagaag atcatcctga gcgagcgcaa gaatgtgcct atcgagtttc cagtgatcga
      841 tc
//
