LOCUS       sequence-53976-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1300_2232
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..89
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatataggat
       61 ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccgac gagctgcacc
      121 taaacttcga cttcaagcac attggctgta aagcaaagga tattattcgt ttcctgcagc
      181 agcgtcttaa gaaggccgtg ccctacaatc gcatgaagct gatgatcgtc gggaacaccg
      241 gttctggaaa gacaaccctc ttgcagcagc ttatgaagac taagaagtcc gacttgggga
      301 tgcagagcgc cactgtgggg atcgacgtga aagactggcc aatccaaatc cgtgacaagc
      361 gcaagcgtga cttagtactg aacgtgtggg actttgccgg cagggaagag ttctactcta
      421 cgcatcctca cttcatgact cagcgtgccc tctatctggc agtttacgac ctctcaaagg
      481 gccaggctga ggtggacgcc atgaaaccct ggctgtttaa catcaaggct cgtgcttcca
      541 gctcccctgt gatcttagtc gggacacact tggatgtttc tgatgagaag cagcgtaagg
      601 catgtatgag taagatcacc aaagagctgt tgaacaaacg cggctttccc gccatccgtg
      661 attaccactt cgtcaacgct accgaggaaa gcgacgcact ggccaagtta cgtaagacca
      721 ttatcaacga aagcctgaac ttcaaaattc gcgaccagct ggtggtcgga cagctgatcc
      781 ccgactgcta cgtggaactt gagaagatca tcctgagcga gcgcaagaat gtgcctatcg
      841 agtt
//
