LOCUS       sequence-53980-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: KLHL7
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
      181 gggtaccgag aacctgtact tccaatccat gggcacaaga cctagaagaa agaaacatga
      241 ctaccgcata gccctatttg gaggctctca accacagtct tgtagatatt ttaacccaaa
      301 ggattatagc tggacagaca tccgctgccc ctttgaaaaa cgaagagatg cagcatgcgt
      361 gttttgggac aatgtagtat acattttggg aggctctcag cttttcccaa taaagcgaat
      421 ggactgctat aatgtagtga aggatagctg gtattcgaaa ctgggtcctc cgacacctcg
      481 agacagcctt gctgcatgtg ctgcagaagg caaaatttat acatctggag gttcagaagt
      541 aggaaactca gctctgtatt tatttgagtg ctatgatacg agaactgaaa gctggcacac
      601 aaagcccagc atgctgaccc agcgctgcag ccatgggatg gtggaagcca atggcctaat
      661 ctatgtttgt ggtggaagtt taggaaacaa tgtttctggg agagtgctta attcctgtga
      721 agtttatgat cctgccacag aaacatggac tgagctgtgt ccaatgattg aagccaggaa
      781 gaatcatggg ctggtatttg taaaagacaa gatatttgct gtgggtggtc agaatggttt
      841 aggtggtctg gacaatgtgg aatattacga tattaagttg aacgaatgga agatggtctc
      901 accaatgcca tggaagggtg taacagtgaa atgtgcagca gttggctcta tagtttatgt
      961 cttggctggt tt
//
