LOCUS       sequence-53981-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2527_M2397T
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             99..116
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctcgcaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
       61 atataggatc tatgcaccac catcaccacc atcaccactt ggtgccccgc ggatccaagc
      121 acattggctg taaagcaaag gatattattc gtttcctgca gcagcgtctt aagaaggccg
      181 tgccctacaa tcgcatgaag ctgatgatcg tcgggaacac cggttctgga aagacaaccc
      241 tcttgcagca gcttatgaag actaagaagt ccgacttggg gatgcagagc gccactgtgg
      301 ggatcgacgt gaaagactgg ccaatccaaa tccgtgacaa gcgcaagcgt gacttagtac
      361 tgaacgtgtg ggactttgcc ggcagggaag agttctactc tacgcatcct cacttcatga
      421 ctcagcgtgc cctctatctg gcagtttacg acctctcaaa gggccaggct gaggtggacg
      481 ccatgaaacc ctggctgttt aacatcaagg ctcgtgcttc cagctcccct gtgatcttag
      541 tcgggacaca cttggatgtt tctgatgaga agcagcgtaa ggcatgtatg agtaagatca
      601 ccaaagagct gttgaacaaa cgcggctttc ccgccatccg tgattaccac ttcgtcaacg
      661 ctaccgagga aagcgacgca ctggccaagt tacgtaagac cattatcaac gaaagcctga
      721 acttcaaaat tcgcgaccag ctggtggtcg gacagctgat ccccgactgc tacgtggaac
      781 ttgagaagat catcctgagc gagcgcaaga atgtgcctat cgagtttcca gtgatcgatc
      841 gcaaacgtct cctccagctc gtgcgtgaga atcagctgca gctcgacgag aacgagctgc
      901 ctcatgctgt gcact
//
