LOCUS       sequence-53982-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZADH2
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
      181 gggtaccgag aacctgtact tccaatccat gcagggctcc gccattcccc aagccatgca
      241 gaagctggtg gtgacccggc tgagccccaa cttccgcgag gccgtcaccc tgagccggga
      301 ctgcccggtg ccgctccccg gggacggaga cctcctcgtc cggaaccgat ttgttggtgt
      361 taacgcatct gacatcaact attcagcagg ccgctatgac ccctcagtta agcctccctt
      421 tgacataggt ttcgaaggca ttggggaggt ggtggcccta ggcctctctg ctagtgccag
      481 atacacagtt ggccaagctg tggcttacat ggcacctggt tcttttgctg agtacacagt
      541 tgtgcctgcc agcattgcaa ctccagtgcc ctcagtgaaa cccgagtatc ttaccctgct
      601 ggtaagtggc accaccgcat acatcagcct gaaagagctc ggaggactgt cggaagggaa
      661 aaaagttttg gtgacagcag cagctggggg aacgggccag tttgccatgc agctttcaaa
      721 gaaggcaaag tgccatgtaa ttggaacctg ctcttctgat gaaaagtctg cttttctgaa
      781 atctcttggc tgtgatcgtc ctatcaacta taaaactgaa cccgtaggta ccgtccttaa
      841 gcaggagtac cctgaaggtg tcgatgtggt ctatgaatct gttgggggag ccatgtttga
      901 cttggctgta gacgccctgg ctacgaaagg gcgcttgata gta
//
