LOCUS       sequence-53983-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAST4
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             187..207
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             564..581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(634..652)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      648..695
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(819..838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg
      181 ggtaccgaga acctgtactt ccaatccatg cagccgatcg tgattcatag cagcggcaaa
      241 aattatggct ttaccattcg cgcgatccgt gtgtatgtgg gcgatagcga tatttatacc
      301 gtgcatcata ttgtgtggaa tgtggaagaa ggcagcccgg cgtgccaggc gggcctgaaa
      361 gccggcgatc tgattaccca tattaacggt gaaccggttc atggcctggt gcataccgaa
      421 gtgattgaac tgctgctgaa aagcggcaac aaagtgagca ttaccaccac cccgtttgaa
      481 aataccgaaa ccagcgtttg acagtaaagg tggatacgga tccgaattcg agctccgtcg
      541 acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      601 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      661 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      721 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      781 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      841 ctttctcgcc acgttcgccg gcttttcccg tcagctctaa tcggggctcc ttag
//
