LOCUS       sequence-53985-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1B3
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             783..803
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             804..821
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             822..845
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             895..912
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgccggcac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ccttaagaag gagatatact atgcacaagc gtccggttcc ggccaaacgc tatgacaacg
      181 tgactattct gttctccggt atcgtagggt ttaatgcatt ttgtagcaaa catgcgtcgg
      241 gcgagggtgc tatgaagatt gttaacctgc tgaatgatct gtacacacgt ttcgatacgc
      301 tgaccgactc tcgcaaaaac ccgtttgtgt ataaagtcga aactgtttgc gataagtata
      361 tgacagtgag tggtctgccg gagccgtgca tccatcacgc ccgttcaatt tgtcatctgg
      421 cactggacat gatggaaatt gcggggcagg tacaggtcga tggcgaaagc gttcaaatca
      481 cgattggtat ccacaccggg gaggtggtaa ctggcgtcat tggtcagcgc atgccgcgtt
      541 actccctgtt cggcaatacg gttaacctga cctcccgcac agaaaccact ggtgaaaaag
      601 ggaaaatcaa tgtgtcggag tatacatacc gttgtctgat gtctccggaa aacagtgacc
      661 cgcaatttca tctggagcac cgcggcccgg taagcatgaa gggtaaaaaa gaaccgatgc
      721 aggtctggtt cctgtcacgt aagaatacgg gcaccgaaga gactaaacag gatgatgacg
      781 cagagaacct ctacttccaa tcgcaccatc atcaccacca tgattacaag gatgacgacg
      841 ataagtgagg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      901 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
//
