LOCUS       sequence-53987-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CLK3
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
      181 gggtaccgag aacctgtact tccaatccat gcagagcagt aagcgcagca gccggagtgt
      241 ggaagatgac aaggagggtc acctggtgtg ccggatcggc gattggctcc aagagcgata
      301 tgagattgtg gggaacctgg gtgaaggcac ctttggcaag gtggtggagt gcttggacca
      361 tgccagaggg aagtctcagg ttgccctgaa gatcatccgc aacgtgggca agtaccggga
      421 ggctgcccgg ctagaaatca acgtgctcaa aaaaatcaag gagaaggaca aagaaaacaa
      481 gttcctgtgt gtcttgatgt ctgactggtt caacttccac ggtcacatgt gcatcgcctt
      541 tgagctcctg ggcaagaaca cctttgagtt cctgaaggag aataacttcc agccttaccc
      601 cctaccacat gtccggcaca tggcctacca gctctgccac gcccttagat ttctgcatga
      661 gaatcagctg acccatacag acttgaaacc agagaacatc ctgtttgtga attctgagtt
      721 tgaaaccctc tacaatgagc acaagagctg tgaggagaag tcagtgaaga acaccagcat
      781 ccgagtggct gactttggca gtgccacatt tgaccatgag caccacacca ccattgtggc
      841 cacccgtcac tatcgcccgc ctgaggtgat ccttgagctg ggctgggcac agccctgtga
      901 cgtctggagc attggctgca ttctctttga gtactaccgg ggcttcacac tcttccagac
      961 ccacga
//
