LOCUS       sequence-53988-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1520_2210
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..90
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             91..108
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatatagga
       61 tctatgcacc accatcacca ccatcaccac ttggtgcccc gcggatccgg acagctgatc
      121 cccgactgct acgtggaact tgagaagatc atcctgagcg agcgcaagaa tgtgcctatc
      181 gagtttccag tgatcgatcg caaacgtctc ctccagctcg tgcgtgagaa tcagctgcag
      241 ctcgacgaga acgagctgcc tcatgctgtg cacttcctta acgagtcagg tgtcctcctg
      301 cacttccagg acccggctct ccagctgagt gacctctatt tcgtggagcc aaagtggctc
      361 tgtaaaatta tggctcaaat cctcactgtc aaggtagagg gctgtccaaa gcacccaaag
      421 ggcatcatct cgcgtcgcga cgtggaaaag tttctctcta agaagcgcaa gtttcccaag
      481 aactacatga gccagtactt taaactgttg gagaagttcc agatcgctct gcctatcggc
      541 gaagagtacc tgttggttcc ttcctccctg tccgatcacc gccccgtgat tgagctgcct
      601 cactgcgaga acagcgagat tatcattcgc ctatacgaga tgccgtactt tccgatgggc
      661 ttctggtcaa ggctcatcaa tcgtctgctt gaaatctccc catatatgct gagcggtcgt
      721 gaacgcgctc tgaggccaaa ccggatgtac tggcgccagg ggatttactt gaactggtcc
      781 cctgaggctt attgtctggt gggctcggaa gtcctggata accaccccga atcattcctg
      841 aagatcaccg ttccttcct
//
