LOCUS       sequence-53993-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1520_2170
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..93
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             94..111
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatata
       61 ggatctatgc accaccatca ccaccatcac cacttggtgc cccgcggatc cggacagctg
      121 atccccgact gctacgtgga acttgagaag atcatcctga gcgagcgcaa gaatgtgcct
      181 atcgagtttc cagtgatcga tcgcaaacgt ctcctccagc tcgtgcgtga gaatcagctg
      241 cagctcgacg agaacgagct gcctcatgct gtgcacttcc ttaacgagtc aggtgtcctc
      301 ctgcacttcc aggacccggc tctccagctg agtgacctct atttcgtgga gccaaagtgg
      361 ctctgtaaaa ttatggctca aatcctcact gtcaaggtag agggctgtcc aaagcaccca
      421 aagggcatca tctcgcgtcg cgacgtggaa aagtttctct ctaagaagcg caagtttccc
      481 aagaactaca tgagccagta ctttaaactg ttggagaagt tccagatcgc tctgcctatc
      541 ggcgaagagt acctgttggt tccttcctcc ctgtccgatc accgccccgt gattgagctg
      601 cctcactgcg agaacagcga gattatcatt cgcctatacg agatgccgta ctttccgatg
      661 ggcttctggt caaggctcat caatcgtctg cttgaaatct ccccatatat gctgagcggt
      721 cgtgaacgcg ctctgaggcc aaaccggatg tactggcgcc aggggattta cttgaactgg
      781 tcccctgagg cttattgtct ggtgggctcg gaagtcctgg ataaccaccc cgaatcattc
      841 ctgaagatca ccgttccttc ctgccgtaag ggttgcattc t
//
