LOCUS       sequence-53999-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1510_2187
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..102
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             103..120
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
       61 ataaatatag gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc
      121 aacttcaaaa ttcgcgacca gctggtggtc ggacagctga tccccgactg ctacgtggaa
      181 cttgagaaga tcatcctgag cgagcgcaag aatgtgccta tcgagtttcc agtgatcgat
      241 cgcaaacgtc tcctccagct cgtgcgtgag aatcagctgc agctcgacga gaacgagctg
      301 cctcatgctg tgcacttcct taacgagtca ggtgtcctcc tgcacttcca ggacccggct
      361 ctccagctga gtgacctcta tttcgtggag ccaaagtggc tctgtaaaat tatggctcaa
      421 atcctcactg tcaaggtaga gggctgtcca aagcacccaa agggcatcat ctcgcgtcgc
      481 gacgtggaaa agtttctctc taagaagcgc aagtttccca agaactacat gagccagtac
      541 tttaaactgt tggagaagtt ccagatcgct ctgcctatcg gcgaagagta cctgttggtt
      601 ccttcctccc tgtccgatca ccgccccgtg attgagctgc ctcactgcga gaacagcgag
      661 attatcattc gcctatacga gatgccgtac tttccgatgg gcttctggtc aaggctcatc
      721 aatcgtctgc ttgaaatctc cccatatatg ctgagcggtc gtgaacgcgc tctgaggcca
      781 aaccggatgt actggcgcca ggggatttac ttgaactggt cccctgaggc ttattgtctg
      841 gtgggctcgg aagtcctgga taaccacccc gaatcattcc tgaagatcac cgttccttcc
      901 tgccgtaagg gttgcattct attgggaca
//
