LOCUS       sequence-54-expo        4812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD30_38
FEATURES             Location/Qualifiers
     source          1..4812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(58..69)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2386..2408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2508..2527
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2605..2623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2691..2795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2796..2864,2865..3656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3014..3033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3827..4415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4316..4335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4569..4586
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4703..4724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4739..4744,4745..4762,4763..4769)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4777..4793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4782..4804
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4801..4812,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4801..4812,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgtcttct tctaaattcc cataaaatcc cgaaactcct
       61 tccctctatc ttctttttct tctcgttttc aaatgtttct ctctatccca ttctctcatc
      121 aattgagtgg gatgaggcta tctctgcctc tcttctgaat ctctgaacca tcttacatta
      181 cactgtggat gacgagcccc acaggctccc ttgcatcaga tactgccatt ggggatggca
      241 aagaagagag aaggtattgt gaggatatat ttttctaaga aaaaacgttt gaagaaaaga
      301 agatgaagaa gatctgcttg attcattgca caagttagaa gtaacagggg tctatatttc
      361 gaagaactta aagggaatgc aactgaacat aaaattaaac aaagggattg aatcctgcag
      421 tgagtatttt cggtttttca ctggttctct gtaaaaagag taatgcaaag ggcaagttaa
      481 cttaggtcgt aaatgtattg aatttgctta aaatctgaag atctagtggt gaaccgtgga
      541 agattatcaa gaggaggctg aagatctgtt taagaaccat taatcaaact ggtattctat
      601 tttcactggt tgtatgtaaa cattctatct tattcctttt atcactgttc tgcactttcc
      661 tataaaaaaa gttgaccgac cgtactctct gaattcattt ttcccgatct taccaactcc
      721 cgatctatct ctatccctgg ttttttcttc gtgctccaat ggaattcttg agacttccac
      781 tatcttctct ggcaccctcc actacgcgta ggcgtctctc gcttcgtgta ttcccgggaa
      841 gccgggttcc cgtctctccc gccgctgccg ctgccgcaca cagctttaca cctcgtagaa
      901 tccccaaaga ggggcgtggc ttgcgggtgc caacatcctc ctgccgagga agaagcaggc
      961 actcatcact cgcatcatca acctcgggat tggccaaagg acccaaaggt atgtttcgaa
     1021 tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc gacggtacca
     1081 tggtattgat atctgagctc cgcatcggcc gctgtcatca gatcgccatc tcgcgcccgt
     1141 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     1201 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     1261 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     1321 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     1381 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     1441 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     1501 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     1561 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     1621 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     1681 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     1741 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     1801 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttaaaatt
     1861 aaacattttt ctaaatttta tatgatttct tttaaatttg caaaaattac ttaaatttga
     1921 attcccgcgc aaatgagtga cttcattttc tgcattattg tgttttccgg ctatattaat
     1981 aggtatttgt ttgtgttttt ctttatttta tgattcgaac tccaatttgt aaattttcga
     2041 acatatttcc ctaaagaaaa aatatgatta atctggaaaa attggaaaat tatttttcaa
     2101 ataaaaaaca aagaaaaaaa tgaagaaaaa cctattagtt tggccataaa acgcaaaaat
     2161 gtcgaaaatg acgtcactca tctgcgcggg aaatcaagaa taattcggcc ttttttattt
     2221 ttttggaaaa tcgtaaaaca tttagaaaaa ttttttaata gttatagtgg gactgtattc
     2281 tgtcatttag ggcaaaagcc agagacgcta ctccaccgtt gggggatcca ctagtcggcc
     2341 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     2401 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     2461 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     2521 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2581 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     2641 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2701 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2761 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2821 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2881 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2941 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3001 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3061 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3121 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3181 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3241 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3301 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3361 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3421 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3481 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3541 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3601 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3661 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3721 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3781 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3841 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3901 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3961 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4021 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4081 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4141 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4201 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4261 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4321 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4381 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4441 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4501 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4561 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4621 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4681 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4741 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4801 caggaaacag ct
//
