LOCUS       sequence-54004-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1500_2187
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatcctta
      121 cgtaagacca ttatcaacga aagcctgaac ttcaaaattc gcgaccagct ggtggtcgga
      181 cagctgatcc ccgactgcta cgtggaactt gagaagatca tcctgagcga gcgcaagaat
      241 gtgcctatcg agtttccagt gatcgatcgc aaacgtctcc tccagctcgt gcgtgagaat
      301 cagctgcagc tcgacgagaa cgagctgcct catgctgtgc acttccttaa cgagtcaggt
      361 gtcctcctgc acttccagga cccggctctc cagctgagtg acctctattt cgtggagcca
      421 aagtggctct gtaaaattat ggctcaaatc ctcactgtca aggtagaggg ctgtccaaag
      481 cacccaaagg gcatcatctc gcgtcgcgac gtggaaaagt ttctctctaa gaagcgcaag
      541 tttcccaaga actacatgag ccagtacttt aaactgttgg agaagttcca gatcgctctg
      601 cctatcggcg aagagtacct gttggttcct tcctccctgt ccgatcaccg ccccgtgatt
      661 gagctgcctc actgcgagaa cagcgagatt atcattcgcc tatacgagat gccgtacttt
      721 ccgatgggct tctggtcaag gctcatcaat cgtctgcttg aaatctcccc atatatgctg
      781 agcggtcgtg aacgcgctct gaggccaaac cggatgtact ggcgccaggg gatttacttg
      841 aactggtccc ctgaggctta ttgtctggtg ggctcggaag tcctggataa ccaccccgaa
      901 tcattcctga agatcaccg
//
