LOCUS       sequence-54008-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1500_2148
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..91
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             92..109
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatatagg
       61 atctatgcac caccatcacc accatcacca cttggtgccc cgcggatcct tacgtaagac
      121 cattatcaac gaaagcctga acttcaaaat tcgcgaccag ctggtggtcg gacagctgat
      181 ccccgactgc tacgtggaac ttgagaagat catcctgagc gagcgcaaga atgtgcctat
      241 cgagtttcca gtgatcgatc gcaaacgtct cctccagctc gtgcgtgaga atcagctgca
      301 gctcgacgag aacgagctgc ctcatgctgt gcacttcctt aacgagtcag gtgtcctcct
      361 gcacttccag gacccggctc tccagctgag tgacctctat ttcgtggagc caaagtggct
      421 ctgtaaaatt atggctcaaa tcctcactgt caaggtagag ggctgtccaa agcacccaaa
      481 gggcatcatc tcgcgtcgcg acgtggaaaa gtttctctct aagaagcgca agtttcccaa
      541 gaactacatg agccagtact ttaaactgtt ggagaagttc cagatcgctc tgcctatcgg
      601 cgaagagtac ctgttggttc cttcctccct gtccgatcac cgccccgtga ttgagctgcc
      661 tcactgcgag aacagcgaga ttatcattcg cctatacgag atgccgtact ttccgatggg
      721 cttctggtca aggctcatca atcgtctgct tgaaatctcc ccatatatgc tgagcggtcg
      781 tgaacgcgct ctgagg
//
