LOCUS       sequence-54010-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1500_2136
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..89
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatataggat
       61 ctatgcacca ccatcaccac catcaccact tggtgccccg cggatcctta cgtaagacca
      121 ttatcaacga aagcctgaac ttcaaaattc gcgaccagct ggtggtcgga cagctgatcc
      181 ccgactgcta cgtggaactt gagaagatca tcctgagcga gcgcaagaat gtgcctatcg
      241 agtttccagt gatcgatcgc aaacgtctcc tccagctcgt gcgtgagaat cagctgcagc
      301 tcgacgagaa cgagctgcct catgctgtgc acttccttaa cgagtcaggt gtcctcctgc
      361 acttccagga cccggctctc cagctgagtg acctctattt cgtggagcca aagtggctct
      421 gtaaaattat ggctcaaatc ctcactgtca aggtagaggg ctgtccaaag cacccaaagg
      481 gcatcatctc gcgtcgcgac gtggaaaagt ttctctctaa gaagcgcaag tttcccaaga
      541 actacatgag ccagtacttt aaactgttgg agaagttcca gatcgctctg cctatcggcg
      601 aagagtacct gttggttcct tcctccctgt ccgatcaccg ccccgtgatt gagctgcctc
      661 actgcgagaa cagcgagatt atcattcgcc tatacgagat gccgtacttt ccgatgggct
      721 tctggtcaag gctcatcaat cgtctgcttg aaatctcccc atatatgctg agcggtcgtg
      781 aacgcgctct gagg
//
