LOCUS       sequence-54018-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1333_2148
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             99..116
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctcgcaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
       61 atataggatc tatgcaccac catcaccacc atcaccactt ggtgccccgc ggatccaatc
      121 gcatgaagct gatgatcgtc gggaacaccg gttctggaaa gacaaccctc ttgcagcagc
      181 ttatgaagac taagaagtcc gacttgggga tgcagagcgc cactgtgggg atcgacgtga
      241 aagactggcc aatccaaatc cgtgacaagc gcaagcgtga cttagtactg aacgtgtggg
      301 actttgccgg cagggaagag ttctactcta cgcatcctca cttcatgact cagcgtgccc
      361 tctatctggc agtttacgac ctctcaaagg gccaggctga ggtggacgcc atgaaaccct
      421 ggctgtttaa catcaaggct cgtgcttcca gctcccctgt gatcttagtc gggacacact
      481 tggatgtttc tgatgagaag cagcgtaagg catgtatgag taagatcacc aaagagctgt
      541 tgaacaaacg cggctttccc gccatccgtg attaccactt cgtcaacgct accgaggaaa
      601 gcgacgcact ggccaagtta cgtaagacca ttatcaacga aagcctgaac ttcaaaattc
      661 gcgaccagct ggtggtcgga cagctgatcc ccgactgcta cgtggaactt gagaagatca
      721 tcctgagcga gcgcaagaat gtgcctatcg agtttccagt gatcgatcgc aaacgtctcc
      781 tccagctcgt gcgtgagaat cagctgcagc tcgacgagaa cgagctgcct catgctgtgc
      841 acttccttaa cgagt
//
