LOCUS       sequence-54020-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1320_2210
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..103
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             104..121
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 accatctcgc aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
       61 tataaatata ggatctatgc accaccatca ccaccatcac cacttggtgc cccgcggatc
      121 ccgtttcctg cagcagcgtc ttaagaaggc cgtgccctac aatcgcatga agctgatgat
      181 cgtcgggaac accggttctg gaaagacaac cctcttgcag cagcttatga agactaagaa
      241 gtccgacttg gggatgcaga gcgccactgt ggggatcgac gtgaaagact ggccaatcca
      301 aatccgtgac aagcgcaagc gtgacttagt actgaacgtg tgggactttg ccggcaggga
      361 agagttctac tctacgcatc ctcacttcat gactcagcgt gccctctatc tggcagttta
      421 cgacctctca aagggccagg ctgaggtgga cgccatgaaa ccctggctgt ttaacatcaa
      481 ggctcgtgct tccagctccc ctgtgatctt agtcgggaca cacttggatg tttctgatga
      541 gaagcagcgt aaggcatgta tgagtaagat caccaaagag ctgttgaaca aacgcggctt
      601 tcccgccatc cgtgattacc acttcgtcaa cgctaccgag gaaagcgacg cactggccaa
      661 gttacgtaag accattatca acgaaagcct gaacttcaaa attcgcgacc agctggtggt
      721 cggacagctg atccccgact gctacgtgga acttgagaag atcatcctga gcgagcgcaa
      781 gaatgtgcct atcgagtttc cagtgatcga tcgcaaacgt ctcctccagc tcgtgcgtga
      841 gaatcagctg cagctcgacg agaacgagct gcctcatgct gtgcacttcc
//
