LOCUS       sequence-54030-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2170
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..101
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             102..119
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catctcgcaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
       61 taaatatagg atctatgcac caccatcacc accatcacca cttggtgccc cgcggatcca
      121 agcacattgg ctgtaaagca aaggatatta ttcgtttcct gcagcagcgt cttaagaagg
      181 ccgtgcccta caatcgcatg aagctgatga tcgtcgggaa caccggttct ggaaagacaa
      241 ccctcttgca gcagcttatg aagactaaga agtccgactt ggggatgcag agcgccactg
      301 tggggatcga cgtgaaagac tggccaatcc aaatccgtga caagcgcaag cgtgacttag
      361 tactgaacgt gtgggacttt gccggcaggg aagagttcta ctctacgcat cctcacttca
      421 tgactcagcg tgccctctat ctggcagttt acgacctctc aaagggccag gctgaggtgg
      481 acgccatgaa accctggctg tttaacatca aggctcgtgc ttccagctcc cctgtgatct
      541 tagtcgggac acacttggat gtttctgatg agaagcagcg taaggcatgt atgagtaaga
      601 tcaccaaaga gctgttgaac aaacgcggct ttcccgccat ccgtgattac cacttcgtca
      661 acgctaccga ggaaagcgac gcactggcca agttacgtaa gaccattatc aacgaaagcc
      721 tgaacttcaa aattcgcgac cagctggtgg tcggacagct gatccccgac tgctacgtgg
      781 aacttgagaa gatcatcctg agcgagcgca agaatgtgcc tatcgagttt ccagtgatcg
      841 atcgcaaacg tctcctccag ctcgtgcgtg agaatcagc
//
