LOCUS       sequence-54032-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2136
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccaag
      121 cacattggct gtaaagcaaa ggatattatt cgtttcctgc agcagcgtct taagaaggcc
      181 gtgccctaca atcgcatgaa gctgatgatc gtcgggaaca ccggttctgg aaagacaacc
      241 ctcttgcagc agcttatgaa gactaagaag tccgacttgg ggatgcagag cgccactgtg
      301 gggatcgacg tgaaagactg gccaatccaa atccgtgaca agcgcaagcg tgacttagta
      361 ctgaacgtgt gggactttgc cggcagggaa gagttctact ctacgcatcc tcacttcatg
      421 actcagcgtg ccctctatct ggcagtttac gacctctcaa agggccaggc tgaggtggac
      481 gccatgaaac cctggctgtt taacatcaag gctcgtgctt ccagctcccc tgtgatctta
      541 gtcgggacac acttggatgt ttctgatgag aagcagcgta aggcatgtat gagtaagatc
      601 accaaagagc tgttgaacaa acgcggcttt cccgccatcc gtgattacca cttcgtcaac
      661 gctaccgagg aaagcgacgc actggccaag ttacgtaaga ccattatcaa cgaaagcctg
      721 aacttcaaaa ttcgcgacca gctggtggtc ggacagctga tccccgactg ctacgtggaa
      781 cttgagaaga tcatcctgag cgagcgcaag aatgtgccta tcgagtttcc agtgatcgat
      841 cgcaaacgtc tcctccagct cgtgcgtgag aatcagctgc agctcgacga gaacgagctg
      901 cctcatgctg tgcacttcct
//
