LOCUS       sequence-54035-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  TITLE     Downward Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBABEneo-H-RAS_V12
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(294..313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(295..313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(341..360)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          368..941
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 gatgtcctca aaagacttgg tgttgttgat ggcaaacaca cacaggaagc cctccccggt
       61 gcgcatgtac tggtcccgca tggcgctgta ctcctcctgg ccggcggtat ccaggatgtc
      121 caacaggcac gtctccccat caatgaccac ctgcttccgg taggaatcct ctatagtggg
      181 gtcgtattcg tccacaaaat ggttctggat cagctggatg gtcagcgcac tcttgcccac
      241 accgacggcg ccaacaacaa caagcttgta ttcggtcata gatcgggaat tcgccctata
      301 gtgagtcgta ttacaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc
      361 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagagggg cccggtttgg
      421 actcagagta ttttatcctc atctcaaaca gtgtatatca ttgtaaccat aaagagaaag
      481 gcaggatgat gaccaggatg tagttgtttc taccaataag aatatttcca cgccagccag
      541 aatttatatg cagaaatatt ctaccttatc atttaattat aacaattgtt ctctaaaact
      601 gtgctgaagt acaatataat ataccctgat tgccttgaaa aaaaagtgat tagagaaagt
      661 acttacaatc tgacaaataa acaaaagtga atttaaaaat tcgttacaaa tgcaagctaa
      721 agtttaacga aaaagttaca gaaaatgaaa agaaaataag aggagacaat ggttgtcaac
      781 agagtagaaa gtgaaagaaa caaaattatc atgagggtcc atggtgatac aagggacatc
      841 ttcccattct aaacaacacc ctgaaaactt tgccccctcc atataacatg aattttacaa
      901 tagcgaaaaa gaaagaacaa tcaagggtcc cccaaactca ccctgaagtt ctcaggatcg
      961 atccggagct ttttgcaaaa agcctaggcc tc
//
