LOCUS       sequence-54042-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PLXNA4A
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             474..491
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(544..562)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      558..605
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(729..748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctaga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcgata aactgattcg tcagcaaatt
      121 gattacaaaa cactggtgct gtcgtgtgtc tcgccggata acgcaaattc tccggaagtt
      181 ccggtaaaaa tcctgaactg cgatacgatt acccaggtga aagagaaaat cctggacgcg
      241 atttttaaaa atgtcccatg tagtcaccgc cctaaagctg ccgatatgga tctggaatgg
      301 cgtcaaggtt caggggcacg catgatcctg caggacgaag atattactac aaaaatcgag
      361 aacgactgga agcgcttaaa taccctggcg cattatcagg ttccggatgg atcggtggta
      421 gctctggtca gcaaacaagt tacgtgatga cgaagcttgc ggccgcactc gagcaccacc
      481 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      541 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      601 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      901 tttcgcttga c
//
