LOCUS       sequence-54052-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RPRD1B
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attcctctag aatattttgt ttactttaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggctcct ccttctctga gtcggcgctg
      121 gagaagaagc tctcggagct gagcaactct cagcacagcg tgcagaccct gtccctttgg
      181 ctcatccacc accgcaagca cgcgggaccc atcgtctccg tgtggcaccg cgagctccgc
      241 aaagccaaat caaatagaaa gcttactttt ctgtatttag cgaatgatgt catccaaaac
      301 agtaaaagga aaggacctga attcactaga gaatttgaat ctgtccttgt ggatgctttt
      361 tctcatgttg ccagagaggc agatgaaggc tgtaaaaaac ctttagaaag attgctgaac
      421 atctggcaag aacgaagtgt gtatggcggc gagttcatac agcagctgaa gctgtctatg
      481 gaggactcca agagccctcc ccccaaagca acagaagaga agaaatctct gaaacgaact
      541 tttcagcaaa ttcaggagga ggaggatgac gactaccctg gcagctactc tcctcaggat
      601 ccttctgcag gacccctctt gactgaggaa ctaatcaaag ctttgcagga tctggaaaat
      661 gccgcatcag gggatgctac tgtccgacag aaaattgctt ctctgcccca ggaagtgcaa
      721 gatgtttctc tattggaaaa aataacagac aaagaggcag ctgaacgtct ttcaaaaaca
      781 gtagatgaag catgtctgtt actagcagaa tataacgggc gcctggcagc agaactggag
      841 gaccgtcgcc agctggctct gatgttggtg gagtataccc agaatcagaa aagatgtttt
      901 gtcgggagaa ggagaaaaaa ctagagggaa tacaaacgaa gctttgcacg a
//
