LOCUS       sequence-54053-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RPRD2
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcgct gcaggtggag gtggaggcag cagtaaggcc
      121 tcctcctcgt cggcctcttc ggcaggggct ctggagtcct cgttggatcg aaaattccag
      181 tcggtaacca acaccatgga gtccattcaa ggcttgtcgt cttggtgtat agagaacaaa
      241 aaacaccaca gtactatcgt ctatcattgg atgaagtggc tccggagatc tgcatatccc
      301 caccgtttga atctctttta ccttgccaat gatgtcatac agaactgtaa aaggaaaaat
      361 gcaatcatat tccgtgaatc atttgctgat gtacttcctg aagcagctgc tctagtgaag
      421 gatccatctg tctctaagtc tgtagaacga atctttaaaa tctgggaaga tagaaatgta
      481 tacccagaag aaatgattgt ggcattgaga gaagctttga gtaccacttt caaaactcag
      541 aagcagctga aagaaaatct gaacaaacaa ccgaataagc agtggaagaa atcacaaaca
      601 tctacaaatc caaaagctgc tctcaagtct aagatagttg ctgaatttcg atctcaggcc
      661 ctaattgaag agctgttgct atacaagcgc tcagaagatc agatagaact gaaggaaaag
      721 cagttgtcaa ctatgagggt ggatgtgtgc agcacagaaa ctctcaaatg cttaaaagat
      781 aaaacaggtg ggaagaagtt ctccaaagaa tttgaagagg caagctccaa gctggaagaa
      841 ttttgtggaa tggattagat aagcaggtga aaaaacggaa ccctcattaa cagaagcact
      901 gga
//
