LOCUS       sequence-54061-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1300_2136
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..102
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             103..120
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
       61 ataaatatag gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc
      121 gacgagctgc acctaaactt cgacttcaag cacattggct gtaaagcaaa ggatattatt
      181 cgtttcctgc agcagcgtct taagaaggcc gtgccctaca atcgcatgaa gctgatgatc
      241 gtcgggaaca ccggttctgg aaagacaacc ctcttgcagc agcttatgaa gactaagaag
      301 tccgacttgg ggatgcagag cgccactgtg gggatcgacg tgaaagactg gccaatccaa
      361 atccgtgaca agcgcaagcg tgacttagta ctgaacgtgt gggactttgc cggcagggaa
      421 gagttctact ctacgcatcc tcacttcatg actcagcgtg ccctctatct ggcagtttac
      481 gacctctcaa agggccaggc tgaggtggac gccatgaaac cctggctgtt taacatcaag
      541 gctcgtgctt ccagctcccc tgtgatctta gtcgggacac acttggatgt ttctgatgag
      601 aagcagcgta aggcatgtat gagtaagatc accaaagagc tgttgaacaa acgcggcttt
      661 cccgccatcc gtgattacca cttcgtcaac gctaccgagg aaagcgacgc actggccaag
      721 ttacgtaaga ccattatcaa cgaaagcctg aacttcaaaa ttcgcgacca gctggtggtc
      781 ggacagctga tccccgactg ctacgtggaa cttgagaaga tcatcctgag cgagcgcaag
      841 aatgtgccta tcgagtttcc agtgatcgat cgcaaacgtc tcc
//
