LOCUS       sequence-54066-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NNMT
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             929..946
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtcattccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
       61 gccatcatca tcatcatcac agcagcggcc tggttccgcg tggtagtatg gaatcaggct
      121 tcacctccaa ggacacctat ctaagccatt ttaaccctcg ggattaccta gaaaaatatt
      181 acaagtttgg ttctaggcac tctgcagaaa gccagattct taagcacctt ctgaaaaatc
      241 ttttcaagat attctgccta gacggtgtga agggagacct gctgattgac atcggctctg
      301 gccccactat ctatcagctc ctctctgctt gtgaatcctt taaggagatc gtcgtcactg
      361 actactcaga ccagaacctg caggagctgg agaagtggct gaaggcagcg ccagcggcct
      421 ttgactggtc cccagtggtg acctatgtgt gtgatcttga agggaacaga gtcaagggtc
      481 cagagaagga ggagaagttg agacaggcgg tcaagcaggt gctgaagtgt gatgtgactc
      541 agagccagcc actgggggcc gtccccttac ccccggctga ctgcgtgctc agcacactgt
      601 gtctggatgc cgcctgccca gacctcccca cctactgcag ggcgctcagg aacctcggca
      661 gcctactgaa gccagggggc ttcctggtga tcatggatgc gctcaagagc agctactaca
      721 tgattggtga gcagaagttc tccagcctcc ccctgggccg ggaggcagta gaggctgctg
      781 tgaaagaggc tggctacaca atcgaatggt ttgaggtgat ctcgcaaagt tattcttcca
      841 ccatggccaa caacgaagga cttttctccc tggtggcgag gaagctgagc agacccctgt
      901 gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactga
//
