LOCUS       sequence-54067-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: KLC2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tgctgtgcca ctctgcaagc aggcactcga
      121 agacctggag aagacgtcag gccacgacca ccctgacgtt gccaccatgc tgaacatcct
      181 ggcactggtc tatcgggatc agaacaagta caaggaggct gcccacctgc tcaatgatgc
      241 tctggccatc cgggagaaaa cactgggcaa ggaccaccca gccgtggctg cgacactaaa
      301 caacctggca gtcctgtatg gcaagagggg caagtacaag gaggctgagc cattgtgcaa
      361 gcgggcactg gagatccggg agaaggtcct gggcaagttt cacccagatg tggccaagca
      421 gctcagcaac ctggccctgc tgtgccagaa ccagggcaaa gctgaggagg tggaatatta
      481 ctatcggcgg gcactggaga tctatgctac acgcctcggg cccgatgacc ccaatgtggc
      541 caagaccaag aacaacctgg cttcctgcta cctgaagcag ggcaagtacc aggatgcgga
      601 gaccttgtac aaggagatcc tcacccgcgc tcatgagaaa gagtttggct ctgtcaatgg
      661 ggacaacaag cccatctgga tgcacgcaga ggagcgggag gaaagcaagg ataagcgccg
      721 ggacagcgcc ccctatgggg aatacggcag ctggtacaag gcctgtaaag tagacagccc
      781 cacagtcaac accaccctgc gcagcttggg ggccctatac cggcgccagg gcaagctgga
      841 agccgcgcac acactagagg actgtgccag ccgtaaccgc aagtgatgac
//
