LOCUS       sequence-54073-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RG9MTD2
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             706..723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(776..794)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      790..837
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtcc aaactcagat ggacatgaca gaaaacgtgt
      121 tcgaagagat gttgttcata gcacccttcg ccttattatt gactgtagtt ttgatcactt
      181 gatggtatta aaggacatta agaaacttca taagcagatt caacgatgtt acgcagaaaa
      241 ccgacgggca ctgcatcctg tgcagtttta cttgacaagc cacggaggcc agctgaaaaa
      301 gaacatggat gaaaatgaca aaggatgggt caactggaag gatatccata tcaaaccaga
      361 gcactatagt gaactcataa agaaagaaga cctgatttac cttacgtcag attcacctaa
      421 tatactgaag gaattagatg aatcaaaggc ctatgtgatt ggaggattag tagatcacaa
      481 ccatcacaag ggactcacat ataaacaagc gtcagattat ggaatcaatc atgcacagct
      541 cccacttgga aattttgtga agatgaatag tcgaaaagtt ttggcagtta atcatgtgtt
      601 tgaaattatt ctggaatacc tggaaacaag agactggcaa gaagcatttt ttactatctt
      661 gccccaacgg aaaggatgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac
      721 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      781 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      841 a
//
