LOCUS       sequence-54078-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PSIP1
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             533..550
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(603..621)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      617..664
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(788..807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcat gactcgcgat ttcaaacctg
      121 gagacctcat cttcgccaag atgaaaggtt atccccattg gccagctcga gtagacgaag
      181 ttcctgatgg agctgtaaag ccacccacaa acaaactacc cattttcttt tttggaactc
      241 atgagactgc ttttttagga ccaaaggata tatttcctta ctcagaaaat aaggaaaagt
      301 atggcaaacc aaataaaaga aaaggtttta atgaaggttt atgggagata gataacaatc
      361 caaaagtgaa attttcaagt caacaggcag caactaaaca atcaaatgca tcatctgatg
      421 ttgaagttga agaaaaggaa actagtgttt caaaggaaga taccgaccat gaagaaaaag
      481 ccagcaatga ggatgtgact aaatgatgac gaagcttgcg gccgcactcg agcaccacca
      541 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      601 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      661 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      781 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      841 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      901 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacgg
//
