LOCUS       sequence-54080-e        1608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tandem dimer of pea ascorbate peroxidase (W41F mutant). This 
            construct has been replaced by APEX2 (construct 49386). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 tdAPX W41F
FEATURES             Location/Qualifiers
     source          1..1608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1585..1608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggcaaga gctatcccac cgttagtccc gattaccaga aggccattga aaaggctaag
       61 aggaagctca gaggttttat cgctgagaag aaatgcgctc ctctaattct ccgtttggca
      121 ttccactctg ctggtacttt tgattccaag acaaagactg gtggtccttt cggaacaatt
      181 aagcaccaag ctgagcttgc tcatggtgct aacaacggtc ttgatatcgc ggttaggctg
      241 ttggagccta ttaaggagca attccctatt gtgagctatg ctgatttcta ccagttggct
      301 ggtgttgttg ctgttgagat taccggtgga cctgaagttc ctttccaccc tggtagggag
      361 gacaagcctg agccaccacc tgagggtcgc ttgcctgatg ccactaaggg ttctgaccat
      421 ttgagggatg tgtttggaaa ggctatgggg cttagtgatc aggacattgt tgctctatct
      481 ggtggtcaca ccattggagc tgcacacaag gagcgttctg gatttgaggg accgtggact
      541 tctaatcctc tcatttttga caactcatat ttcactgagt tgttgactgg tgagaaggat
      601 ggccttcttc agttgccaag tgataaggca cttttgactg actctgtatt ccgccctctt
      661 gttgagaaat atgctgcgga tgaagatgtt ttctttgctg attatgctga agcacatctt
      721 aagctctctg agctgggctt cgccgaggct ggctccacct ctggttctgg caaaccgggc
      781 tccggtgaag gctctaccaa aggttctggc gggactggcg ggagcaagga tccaagggga
      841 aaatcttacc caactgttag tcccgattac cagaaggcca ttgaaaaggc taagaggaag
      901 ctcagaggtt ttatcgctga gaagaaatgc gctcctctaa ttctccgttt ggcattccac
      961 tctgctggta cttttgattc caagacaaag actggtggtc ctttcggaac aattaagcac
     1021 caagctgagc ttgctcatgg tgctaacaac ggtcttgata tcgcggttag gctgttggag
     1081 cctattaagg agcaattccc tattgtgagc tatgctgatt tctaccagtt ggctggtgtt
     1141 gttgctgttg agattaccgg tggacctgaa gttcctttcc accctggtag ggaggacaag
     1201 cctgagccac cacctgaggg tcgcttgcct gatgccacta agggttctga ccatttgagg
     1261 gatgtgtttg gaaaggctat ggggcttagt gatcaggaca ttgttgctct atctggtggt
     1321 cacaccattg gagctgcaca caaggagcgt tctggatttg agggaccgtg gacttctaat
     1381 cctctcattt ttgacaactc atatttcact gagttgttga ctggtgagaa ggatggcctt
     1441 cttcagttgc caagtgataa ggcacttttg actgactctg tattccgccc tcttgttgag
     1501 aaatatgctg cggatgaaga tgttttcttt gctgattatg ctgaagcaca tcttaagctc
     1561 tctgagcttg gatttgctga agccgactac aaggatgacg acgataag
//
