LOCUS       sequence-54081-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NCOR2
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             332..349
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(402..420)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      416..463
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      500..955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(587..606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     797..818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggct ggactgagga agagatgggg acagcaaaga
      121 aaggcctcct ggaacatggg aggaactggt cagccattgc ccgcatggtg ggctccaaga
      181 ccgtgtccca gtgtaagaac ttctacttca actacaagaa gaggcagaac ctggacgaaa
      241 tccttcagca gcacaagcta aagatggaga aggagaggaa cgctcggagg aagaagaaga
      301 agtgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      361 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      421 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      481 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
      961 gcactt
//
