LOCUS       sequence-54082-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(178..195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(429..448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actcgagcaa agcccgccga aaggcgggct tttctgtgtc gaccgatgcc cttgagagcc
       61 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
      121 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
      181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      781 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      841 agttggtagc tcttgatcc
//
