LOCUS       sequence-54100-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SETD7
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             813..830
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             860..877
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca gtataaagat aacattcgtc
       61 atggagtgtg ctggatatat tacccagatg gaggaagcct tgtaggagaa gtaaatgaag
      121 atggggagat gactggagag aagatagcct atgtgtaccc tgatgagagg accgcacttt
      181 atgggaaatt tattgatgga gagatgatag aaggcaaact ggctaccctt atgtccactg
      241 aagaagggag gcctcacttt gaactgatgc ctggaaattc agtgtaccac tttgataagt
      301 cgacttcatc ttgcatttct accaatgctc ttcttccaga tccttatgaa tcagaaaggg
      361 tttatgttgc tgaatctctt atttccagtg ctggagaagg acttttttca aaggtagctg
      421 tgggacctaa tactgttatg tctttttata atggagttcg aattacacac caagaggttg
      481 acagcaggga ctgggccctt aatgggaaca ccctctccct tgatgaagaa acggtcattg
      541 atgtgcctga gccctataac cacgtatcca agtactgtgc ctccttggga cacaaggcaa
      601 atcactcctt cactccaaac tgcatctacg atatgtttgt ccacccccgt tttgggccca
      661 tcaaatgcat ccgcaccctg agagcagtgg aggccgatga agagctcacc gttgcctatg
      721 gctatgacca cagccccccc gggaagagtg ggcctgaagc ccctgagtgg taccaggtgg
      781 agctgaaggc cttccaggcc acccagcaaa agcatcatca tcatcatcac tgatgacgaa
      841 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      901 cccgaaagga agctgagttg gctgctgcca ccgctgagca taactagcat aac
//
