LOCUS       sequence-54102-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PLXNB2
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             458..475
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(528..546)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      542..589
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(713..732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcct gctgggcgat gacgttgagt
      121 acgcgccact gacagtatct gttatcgtcc aagatgaagg tgttgacgcc atccctgtga
      181 aagtactgaa ttgtgatacg attagtcagg tcaaagaaaa aatcattgat caagtttatc
      241 gtggccagcc gtgctcatgt tggccgcgcc cagactccgt ggtactggag tggcgtcctg
      301 gttcgaccgc acagatcctg tcggatctgg acctgacttc tcaacgcgaa gggcgttgga
      361 aacgcgttaa cacactgatg cactacaatg tgcgtgatgg agctaccctg attctgagta
      421 aagtcggctg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      481 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      541 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      601 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      841 tagggtgatg gttcacgtag tgggccatcg cccctgatag acgggttttt cgcctttgac
      901 gtggatcacg tct
//
