LOCUS       sequence-54117-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG545
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    160..259
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(261..305)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(318..338)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      121 agagattttg agacacgggc cagagctgca gctggatggc aaataatgat tttattttga
      181 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact
      241 ttgtacaaga aagctgggtg gctgtccatg tgctggcgtt cgaatttagc agcagcggtt
      301 tctttgaatt tggcggcgcc ctgaaaataa agattctctc tcgagcggcc gccaccggtg
      361 gagctccacc gatcccccgg gcaccatgct tgacttggta gaggactgta tcttgcaact
      421 gcgtgttcgt atagcttaac tttgttgtaa tccgaatcgt ttcctagtat ttcaattctg
      481 tgatcaacaa agtgtgcatc tggaagttgg acgaccttct ttgctttgta agtagtttta
      541 aaatcgcaaa ggtagtgacc tccaccttct agtagtagtg ccatgttaat gttctgaaaa
      601 tttaaatata atccgttagt tagtaagtcc aagtttaaac ttactccaac aagtagtcca
      661 tctctaacat gtagtttttc agtcgatggt tcccacttaa gagtcttctt ttgcataact
      721 ggtccgttag gaggaa
//
