LOCUS       sequence-54132-e       11974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Fields RL, House SB, Gainer H
  TITLE     Regulatory domains in the intergenic region of the oxytocin and 
            vasopressin genes that control their hypothalamus-specific 
            expression in vitro.
  JOURNAL   J Neurosci. 2003 Aug 27;23(21):7801-9.
  PUBMED    12944509
REFERENCE   2  (bases 1 to 11974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2003-08-27"; volume: "23(21)"; pages: "7801-9"
COMMENT     Sequence Label: p3.5VPIII.EGFP.IGR2.1
FEATURES             Location/Qualifiers
     source          1..11974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(202..207,208..224,225..231)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    239..255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     244..266
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     regulatory      5738..5747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5744..5746,5747..5749,5750..6463)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5744..5746,5747..5749,5750..6460)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5789..5810)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6050..6069)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6397..6418
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8740..8757)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8910..8996
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9088..9115
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        9135..9226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9227..9295,9296..10087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9445..9464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10258..10846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10747..10766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11000..11017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11032..11172
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11098..11117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11258..11280
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11274..11465)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag
       61 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
      121 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
      181 caaatattct gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggaatt
      241 gtgagcggat aacaatttca cacaggaaac agaccatggc tggtgaccac gtcgtggaat
      301 gccttcgaat tcagcacctg cacatgggac gtcgacctga ggtaattata acccgggccc
      361 tatatatgga tccccacacc gtgtgcatat ggttcaccac ctttttcagt gacttcctct
      421 ttgcagtcac cagctgaagg tatggggagg actctagcgg ccgttggcct ctacccagcc
      481 gggacctgca aggccgtgca cagcctcatc cctcagcaat ggcactgagt gatgccaggc
      541 ctgccatctc atgtgccttt ggctgttggg acacagtgtg cctctatgga gccagcctaa
      601 cccttcgtgc tagacaaact gcacaatttc tgctcgacct cagaggcttt tttatctttc
      661 cttgtgagtt tcttgtcacc cagcagtgtt tcttgcctct ttgcttgatg cctctgcggg
      721 aagatggaca gggctggttt ttcccacttc cccgcaaaca ggtttgtcat accttttggg
      781 ggtgggtggt tttgtccttg tcaagcatag caggtcaccc gacccagcac tgtgaatcta
      841 cccaactcgt ctctgtacaa aattaaacaa caaaaagaaa gttgtttttc tcgagacgag
      901 gtttctctat gtagccctgg ctatcctgga tcttgctcta tagaccaggc tatcctcgaa
      961 ctcagagaac tgcctgcctc tgtctcttga gtgctggcat tgagtattaa gtcgtgggag
     1021 gtgcagctat ttgaatagtg attgcagatt ggaaccctga gaaggtgggt gggtgggtgg
     1081 gggaacaaga gttaaatcaa actatgttaa atcagaaagg ccccatgcct atccagtgga
     1141 agctgtcact ggaggttcta actacaggtc tggcatgtca gggaagggct cagaaggttt
     1201 gagctggggc atcattagtt tgtaaaaatt atgaaaatac tctaggaaga agacaagaga
     1261 agggacacca tagcaagcaa actttgggat gttccaggat tgtgaagtca gaaaatgaag
     1321 caggggggtg gtgtggcaga aggtccagga gagcacagag tgtgctgtca aaacaaaggc
     1381 ttcatcgtgt gaaacttcac tgggaaagat ggacacgccc acaaagctct tctgattaca
     1441 acggtagtaa tttcagtagg cagatttggg cagaaatcgg aatgcagggg attacagagt
     1501 aaatgctgac ttttggataa gaataacaga tcacatgaca ggcatggtgg ctcacatctt
     1561 tgaaagcaac actcccaagg cagagttagg ctgtgagctt gaggctagtc tggtctacat
     1621 agaaatttcc agtccagcca gagctacaga ttcagaccct gtcagtattt gggaaaaaga
     1681 agctatgagg ctatctggga gtccatgtgg catgtggcga gggaggacct gaactgaaga
     1741 gagccagcat gctgtcggga cctttcctag ccagcagaga tgctaaggca ggtgaagatt
     1801 aggtctcgtg gacctgacat ccattcatga agcagcacag agccctcaaa gctctggtgg
     1861 acatgccact caagggctct ggacaggccc agacaatagt ctgccccaac gctcatcttc
     1921 agtccattag tggaaaaaag gtttcttctg accaaggaag ctgtttcctc tggaaaacaa
     1981 tcacttaaca aggacattca taacacaaag ccgctgtcag ctaacatcac catgacgcaa
     2041 gcacttccct tggggttcat agtaagtgac tcagggctca cagccctgca gagtgcttgc
     2101 ccttcactac tttatgctgg agttaagtgg ggagtcagac accccagatg acctgcccaa
     2161 gtcagaaagc ttcgaggcat agaagccagc atgtcctcgg ccttcctaca tagcagctgc
     2221 ctcttatcct caagtgccac ccagtcattc ctgaaaaggt ctggccgcca ctcctgagga
     2281 tggacgggca gcttggctcc ctgtccccac tgtacttgtt gggaagaggc caaacagtcc
     2341 tctggagctc ttgaagcatg ctggtgtacc cgggagggtg gcaagagcga tggggatggg
     2401 agggccctgg ctatgcatgg tctagaagcc gtgggctagg tgtgcatagg tcaagcatag
     2461 gccaactaat ctgggcccca aaccataaag tttttctggt gcctgaagta aaggagtctg
     2521 taaagcctag agcagaatgc ggtgatgcac gcctgtaacc ctagccactg gagaggcaga
     2581 gacaaaaaga tgaagagctc aaagcagctt tcagctgtag taagttcaag gccagcctgg
     2641 actaaactag actgccttag aaacaaacaa ctgacttaca gtctaaatca ggaattacac
     2701 cagctttctc agactggctc tgtttagctg ggtctcctcc catttcctgt cctagaataa
     2761 cacccacttc caatcctgcc cttagacctg agatattccc aacctcaggg cctggggtct
     2821 ccccaaagct ctctttcctc tttacggctg tgggtctcac caaggactat ctctgagctc
     2881 tatcctacac cctagcctct accctagaag gcctggaact cacagaaact ctcctgcctc
     2941 tgcttccaat ggctgggact aaaggcacgc gtcacgactg gccttctttt ttatgttttt
     3001 taatattgag acagtgtctc agccaagtta ctcagtctgg ccttgaactt ttgacctttc
     3061 tactttagct tcccaagtgg ctctgaccac aggcccatga agccagatcc tctgtgcaag
     3121 ctttgcacct tactgagcct ggagttaaca ttgccaccat agctttccca tttgtgtcct
     3181 tagtagaaac aatgggcact attcccaagc tctccccacc cccggctcta cagggttatg
     3241 catgggatag aaacatcctg ggtgcccccg aagcagccat gcctggcaca gggcagacct
     3301 ttcactgtgt tcagccttga ctccatgact gtggccgtta gcccatgaga ctgcaagtgg
     3361 gaatttcttt ctaaagccca cctgatatgg gtgcttcctc tcatcctata ccacaactaa
     3421 caacctgccc accccttctg gtgctgaccc tgctccatac gtgccagccc ttgctggatg
     3481 ggggcctgtg gaccccttta gtctgctgag agcagctgga atattcaact atgatttcca
     3541 ggtgaccctc cagtcggctc acctcactga tcgcacagca ccaatcactg tgggcagtgg
     3601 ctcctgtcag acggtggccg gtgacagcct gcatggctgg ctcccctcct ccaccaccct
     3661 ctgcactgac acgcccacgt gtgtccccag atgcctgaat cactgctgac agcttgggac
     3721 ctgtcggctg aggctcctgg ggagccactg gggagggggt tagcagccac gttgtcgcct
     3781 cctagggaac acctgcagac ataaataggc agcccagccc gccaaggcag cagagcctga
     3841 gctgcacaca gtgcccacct atgctcgcca ggatgctcaa cactacgctc tccgcttgtt
     3901 tcctgagcct gctggccttc tcctccgcct gctacttcca gaactgccca agaggcggca
     3961 agagggccat ctctgacatg gagctgagac aggtaccact gtggtccctt taggctgctg
     4021 gcagatgctg tagggacagg ggctaggaga gagggaaatg ttatctgagc agtcagactt
     4081 tatgggaggt tcctggaagg aggcagtatc ttacagcaga gtagatggac tacccagaag
     4141 ggtgagaggg gaccaggtgc tagagaagcc gcataaagga tactgtcccc aggcagggga
     4201 tatgccagaa aatgagagac acttccttat gactgggctt gggatgagaa caggttaaac
     4261 tgggtgccct ggactcctct gcacacccgg aggttgagga ctgggcagat tatgcaaaac
     4321 tattcttgcc tgaattcaaa tcctttccca cccagctcag cctcccttgg tgccttttct
     4381 agccagcagt gccagcttct tcctgtccac agaaggtggc caatgcccca tgcccaagtg
     4441 gagcatttcc cgcatcgaac ctcagcctct tgctcagatc tgttgtattg tatgttcagc
     4501 tgtgagtctg cctgcccctc tggcagagtt tgagggaatc tagctactag gctcaattct
     4561 ggtcaggcga tgggtggctg aatgttgagt tgttgaacaa gtttcgagtg ggcaggtagg
     4621 cagctcctgt agtctgcctt ccctttgcag agttcctttg gaggtgtgtc cgggacctaa
     4681 tttagtcctt gccacctacc aactaagaca ttataggttg gcgggaggta aaggctcata
     4741 tgaagccacc agcgtggggc agaggtgaga gcaaagccag aagacgagtg agctatctag
     4801 atactctgtg gggagtgaga atctagggat gtgtaggagg accatctgaa tgacggagag
     4861 gtaagcctcc gagagatggc tgcacaccag tgacactgag aactgaggaa ggtctccctc
     4921 aagtgttgcc ccgcagcgag agggttttga gacctcatga gctgaccact gatctttctg
     4981 atgtcccagc cggttagatt ttcactcttg cccttaccgc tgcttcgtcc tggacatcgc
     5041 cagagcacca gcaacgcaaa gcagcaggtg acactaggtt cccaccgccc ctcttggcct
     5101 cgttccagct gacctccccc acccctttcc tccacagtgt ctcccctgcg gcccgggcgg
     5161 caaaggacgc tgcttcggac caagcatctg ctgcgcggac gagctgggct gcttcgtggg
     5221 caccgccgag gcgctgcgct gccaggagga gaactacctg ccctcgccct gccagtccgg
     5281 ccagaagccc tgcgggagcg ggggccgctg cgccgccgtg ggcatctgct gcagcgacgg
     5341 tgcgcacagg gccgggcggg catcgcatgg agtggggtgg gaggcaaagg cggggggcta
     5401 agtggggggc tggggaatca ggacaggaag tggagggtga gaatgaaggg agtcgagggt
     5461 tggaaggtag caggggggat tgttgggacc gcacccttgg agctgagccc aaggcgcctg
     5521 cgctcacaga gctcttcctt cagagagctg cgtggccgag cccgagtgcc acgacggttt
     5581 tttccgcctc acccgcgctc gggagccaag caacgccaca cagctggacg gccctgctcg
     5641 ggcgctgctg ctaaggctgg tacagctggc tgggacacgg gagtccgtgg attctgccaa
     5701 gccccgggtc ttgcggcccg ggatccaccg gccggtcgcc accatggtga gcaagggcga
     5761 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5821 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5881 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5941 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6001 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6061 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6121 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6181 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6241 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6301 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6361 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6421 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcaactactg
     6481 agccatcgcc cccacgcctc gcccctacag catggaaaat aaacttttaa aaactgcacc
     6541 ctggtgtctg tctctatttc tggggtgggg agaagagggg agggagaggc gttggagtgg
     6601 gaactttcta ctctgccctg gctaaccccc acacccgaag tcgtgcatag tccacgcccc
     6661 cttccccacc agaaggggca gaacctgtaa gtcttataga ataaaggaag cgatcgggtg
     6721 ctcctggata cccacctaat actcaggaca aaggcaaccc caagctcaga agaggggaaa
     6781 ggtctgggac ccaaagcggc cctgaagggt cagggtgact tactgattct ttggctgaga
     6841 ccggaataaa aggagagagg caggccattg gtggacaaaa ggaactgagc tggatatggg
     6901 gagagacaga tcggcaagag taacaaagaa agggggggac aagccagggg tggctatctc
     6961 agccctgatt tcacctaagg caaaaacagc ttgaaggact tccactcacc caagactgtg
     7021 cttggatggg agaaggtaca gagatgttac cccaaaccta aaggagacaa cgccacctgc
     7081 attctctcca ggtgctccac accaaccact gagaggcaaa ccccagtgag agcctagcta
     7141 caagcccctt acaggcacgt gttcaaactg ccacgccctt actccccgca cggaaataga
     7201 caagataggc tggagttgtg tctcaggagc agaaggcatc cctggcctga atttggtccc
     7261 cagcaccatg gagatggaag aggaggggaa aggggaagag gaggaaaggg taacaactgc
     7321 taggggcgtt tctatgactc ttgaccgcaa ccatagctcg ggactcagag atgcatgagc
     7381 ccagcagcag cgcgcacaca tgctgacagt agcgcccaga ccacgctctt tggcactcct
     7441 tccctcccct tctcattagc ttctcttttt tttctaaaat tttagattta ttcatttatt
     7501 tatgtatatg gatgcttttt catgtatata tgtatgtatg atctgggcat catgtgtgtg
     7561 cctggtgctg cagaggtcag gagagggcat cagactccct ggacctggag ttacaggtag
     7621 ttacatccta ccatgtgggc actatgaact gaatcccagt cctcttaaat gaccttaacg
     7681 accaagtcag cgagcacttc agcccccaac ctgtccttcc tccaggcccc ttggtgcctt
     7741 ggccagagag ggctgctctc tcaggttttc catccttcca tcttctgggc tccctgttct
     7801 tcaccctcct gttaacttcc cactttctct ctccgcccca tcagtcccgg gcagcctctt
     7861 ctcctaagac tgcagcaagg tctcctaagg ccacactatg ctataacttc ttgggatgct
     7921 ctgcagactc taaggtcagg ggcaactaca gggaagaaag agattagtct cagtgacctg
     7981 actaggtggc tcaggatggt cagttctgga cagagtcttg gtcttctacc cggtctccag
     8041 ttctcttccc acctcctcag cacctttccc cctctctgct tgcctgggtt taaccagggg
     8101 gtacagggac caggcagact cctatcccag cctcctcctt acatggccct ctcagggtct
     8161 gacctccaat agggcagggc caaggaaggg cgaggccaaa aagggacaga gaggaggtag
     8221 gtgtggcagg ttgtgtggcc acacttggtt caatccctag ggctggtgta tggacagttt
     8281 cagaatggag gtgagagcct cactctgctc ctctgcccag gtggagactt gcaaacgtcc
     8341 ctttccaaca cctgtttctg gaagaatcag gctcctattt ctcaaggaaa ggagttgcat
     8401 gtgcccagcc tacttgtgcc tactggacat gctattcttc ttccctttca gggagtccct
     8461 tcctcagcta cagagccatt gaagggcact ccgagacatc acagtagttg cttgccgcct
     8521 ctctgcagcc tctagaagca aaggggcttg tgtgagcaaa tgccaggagt gctctgaccc
     8581 ccacacagat gctgtggcac gcgtacaacc cccaaacaca cagcgaagca atagtgaaaa
     8641 gtcgaccgcg gacatgtaca gagctcgaga agtactagtg gccacgtggg ccgtgcacct
     8701 taagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     8761 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     8821 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     8881 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     8941 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     9001 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     9061 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     9121 ttctacaaac tctttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     9481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    10021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    10081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10321 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10381 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
    10441 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10501 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10561 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10621 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10681 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10741 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10801 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10861 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10921 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10981 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
    11041 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
    11101 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
    11161 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
    11221 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    11281 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
    11341 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
    11401 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
    11461 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
    11521 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
    11581 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
    11641 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
    11701 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
    11761 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
    11821 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
    11881 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac
    11941 ccaacgctgc ccgaaattct catgtttgac agct
//
