LOCUS       sequence-54137-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3.1/Puro-CAG-VSFP-CR
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
       61 ccctggctca caaataccac tgagatctgc atgagctagc gccaccatgg agggattcga
      121 cggttcagat tttagtcctc cagctgattt agttggcgtt gacggtgcag tcatgcggaa
      181 cgtcgttgac gtcacgataa atggtgacgt cactgctccg ccgaaagctg cgccaagaaa
      241 aagtgaatcg gtaaagaaag ttcattggaa tgatgtagac caaggaccga gtgaaaaacc
      301 agagacaaga caggaggaac gaatagatat acccgagatt tcaggtctat ggtggggcga
      361 gaatgaacat ggagtggacg atgggagaat ggagatacct actactggtg taggtcgcgt
      421 ccagtttcgt gtccgagcag tgattgatca tctagggatg cgagtctttg gagtcttcct
      481 aattttcttg gacatcatcc tcatgatcat tgatctcagt cttccaggaa aaagtgaatc
      541 ttcacaatcc ttttatgacg ggatggcttt ggctctttct tgttatttca tgctggattt
      601 aggattaagg atatttgcct acgggcccaa gaatttcttc accaacccct gggaggttgc
      661 tgatggtttg attatcgttg tcacattcgt cgtcacgata ttttacactg tgttagatga
      721 atactg
//
