LOCUS       sequence-54138-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CBLC
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 accttaagaa ggagatatac tatggggcga cagtgggaag aggcccgcgc cctgggccgg
      181 gcagtcagga tgctgcagcg cctagaagag caatgcgtcg acccccggct gtccgtgagt
      241 cccccttcgc tgcgggacct gctgccccgc acagcgcagc tgcttcgaga ggtggcccat
      301 tctcggcggg aggccggcgg aggcggcccc gggggtcccg gcggctctgg ggactttcta
      361 ctcatctacc tggccaatct ggaggccaag agcaggcagg tggccgcgct gctgcctccc
      421 cggggccgaa ggagtgccaa cgacgagctc ttccgggcgg gctccagact caggcgacag
      481 ctggccaagc tggccatcat cttcagccac atgcacgcag agctgcacgc actcttcccc
      541 gggggaaagt actgtggaca catgtaccag ctcaccaagg cccccgccca caccttctgg
      601 agggaaagtt gcggagcccg gtgtgtgctg ccctgggctg agtttgagtc cctcctgggc
      661 acctgccacc ctgtggaacc aggctgcaca gccctggcct tgcgcaccac cattgacctc
      721 acctgcagcg ggcacgtgtc catcttcgag ttcgacgtct tcaccaggct ctttcagcca
      781 tggccaacac tcctcaagaa ctggcagctc ctggcagtca accacccagg ctacatggcc
      841 ttcctcacct atgatgaggt ccaagagcgt ctgcaggcct gcagggacaa gccaggcagt
      901 tacat
//
