LOCUS       sequence-54139-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TP73
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             748..768
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             769..786
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             787..810
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             860..877
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 accttaagaa ggagatatac tatggcgcct gtcatcccct ccaacaccga ctaccccgga
      181 ccccaccact ttgaggtcac tttccagcag tccagcacgg ccaagtcagc cacctggacg
      241 tactccccgc tcttgaagaa actctactgc cagatcgcca agacatgccc catccagatc
      301 aaggtgtcca ccccgccacc cccaggcact gccatccggg ccatgcctgt ttacaagaaa
      361 gcggagcacg tgaccgacgt cgtgaaacgc tgccccaacc acgagctcgg gagggacttc
      421 aacgaaggac agtctgctcc agccagccac ctcatccgcg tggaaggcaa taatctctcg
      481 cagtatgtgg atgaccctgt caccggcagg cagagcgtcg tggtgcccta tgagccacca
      541 caggtgggga cggaattcac caccatcctg tacaacttca tgtgtaacag cagctgtgta
      601 gggggcatga accggcggcc catcctcatc atcatcaccc tggagatgcg ggatgggcag
      661 gtgctgggcc gccggtcctt tgagggccgc atctgcgcct gtcctggccg cgaccgaaaa
      721 gctgatgagg accactaccg ggaggcagag aacctctact tccaatcgca ccatcatcac
      781 caccatgatt acaaggatga cgacgataag tgaggatccg aattcgagct ccgtcgacaa
      841 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      901 cccgaaagga agctgagttg gctgctgcac gctgacatac ta
//
