LOCUS       sequence-54146-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII415
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..22)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    64..567
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        604..708
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg
       61 acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg
      121 gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga aagtaaataa
      181 agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca
      241 acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta aatagatata
      301 cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca
      361 cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt
      421 agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt
      481 tctttaattt ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa
      541 attataatta tttttatagc acgtgatgaa aaggacccag gtggcacttt tcggggaaat
      601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      781 ccagaaacgc tg
//
