LOCUS       sequence-54167-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII428
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..22)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagt
       61 atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt
      121 ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga
      181 atgggataat atcacaggag gtactagact acctttcatc ctacataaat agacgcatat
      241 aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca
      301 ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg
      361 cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt
      421 ctctagctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga
      481 cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat
      541 accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc
      601 ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac
      661 attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt
      721 catagagtga atcgaa
//
