LOCUS       sequence-54185-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, 
            Munoz-Pinedo C, Green DR
  TITLE     Resistance to caspase-independent cell death requires persistence of
            intact mitochondria.
  JOURNAL   Dev Cell. 2010 May 18;18(5):802-13.
  PUBMED    20493813
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2010-05-18"; volume: "18(5)"; pages: "802-13"
COMMENT     Sequence Label: pcDNA3-Smac(1-60)mCherry
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             187..894
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(329..347)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     597..616
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggcggctc tgaagagttg gctgtcgcgc agcgtaactt cattcttcag gtacagacag
       61 tgtttgtgtg ttcctgttgt ggctaacttt aagaagcggt gtttctcaga attgataaga
      121 ccatggcaca aaactgtgac gattggcttt ggagtaaccc tgtgtgcggt tcctattgca
      181 gaattcgtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      241 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      301 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      361 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      421 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      481 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      541 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      601 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      661 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      721 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      781 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      841 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaa
//
